Ribosomal L12 stalks recruit elongation factors to speed protein synthesis in Escherichia coli

JL Hofmann and TS Yang and AM Sunol and RN Zia, COMMUNICATIONS BIOLOGY, 8, 940 (2025).

DOI: 10.1038/s42003-025-08366-4

Translating ribosomes must wait after each elongation step for a new ternary complex (EF-Tu & sdot; aa-tRNA & sdot; GTP) to arrive, facilitating rapid codon recognition testing. We recently showed that this wait-time rate-limits elongation in Escherichia coli due to competitive combinatoric searching through crowded cytoplasm by thousands of E. coli's 42 unique ternary complexes. Here, we investigate whether ribosomal L12 subunits pool translation molecules to reduce this wait time. We mimic transport and reactions underlying elongation in a physiologically accurate, physically-resolved model of crowded cytoplasm. We find that L12 pre-loading as much as doubles translation rate by reducing diffusive search time. But more L12 is not always better: faster-growing bacteria tend to have fewer L12. We resolve this apparent contradiction by demonstrating tradeoffs between binding and novel sampling as a function of copy number in E. coli. Variable L12 copy numbers may thus have evolved for fast or slow bacterial growth as complementary survival strategies.

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