Molecular interactions underlying the phase separation of HP1 alpha: role of phosphorylation, ligand and nucleic acid binding
C Her and TM Phan and N Jovic and U Kapoor and BE Ackermann and A Rizuan and YC Kim and J Mittal and GT Debelouchina, NUCLEIC ACIDS RESEARCH, 50, 12702-12722 (2022).
Heterochromatin protein 1 alpha (HP1 alpha) is a crucial element of chromatin organization. It has been proposed that HP1 alpha functions through liquid-liquid phase separation (LLPS), which allows it to compact chromatin into transcriptionally repressed heterochromatin regions. In vitro, HP1 alpha can undergo phase separation upon phosphorylation of its N-terminus extension (NTE) and/or through interactions with DNA and chromatin. Here, we combine computational and experimental approaches to elucidate the molecular interactions that drive these processes. In phosphorylation-driven LLPS, HP1 alpha can exchange intradimer hinge-NTE interactions with interdimer contacts, which also leads to a structural change from a compacted to an extended HP1 alpha dimer conformation. This process can be enhanced by the presence of positively charged HP1 alpha peptide ligands and disrupted by the addition of negatively charged or neutral peptides. In DNA-driven LLPS, both positively and negatively charged peptide ligands can perturb phase separation. Our findings demonstrate the importance of electrostatic interactions in HP1 alpha LLPS where binding partners can modulate the overall charge of the droplets and screen or enhance hinge region interactions through specific and non-specific effects. Our study illuminates the complex molecular framework that can fine-tune the properties of HP1 alpha and that can contribute to heterochromatin regulation and function.
Return to Publications page